rhaEW

168

bifunctional-rhamnulose-phosphate aldolase/L-lactate dehydrogenase

Locus: BSU_31220

Molecular weight: 75.84 kDa

Isoelectric point: 5.78

Protein length: 689 aa

Gene length: 2070 bp

Function: utilizatio of rhamnose and rhamnogalacturonan (pectin)

Product: bifunctional-rhamnulose-phosphate aldolase/L-lactate dehydrogenase

Essential: no

Synonyms: rhaEW, yulA, yuwG, yuxG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3347 (Galperin et al., 2021)

Gene

Coordinates: 3,201,860 → 3,203,929

The protein

Catalyzed reaction/ biological activity

• rhamnulose-1-phosphate --> dihydroxyacetone-phosphate + L-lactate PubMed

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Cofactors

• NAD PubMed

Structure

• P40747 (AlphaFold)

Expression and Regulation

Operons

• Genes: rhaEW-rhaR-rhaB-rhaM-rhaA

  Description: PubMed

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed

  Regulatory mechanism

  • RhaR: repression, PubMed, in rhaR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A638 (yuxG::erm), available at the NBRP B. subtilis, Japan
• BKE31220 (ΔrhaEW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTGATATTCCTCCAT,  downstream forward: _UP4_TAGATATATGCTATGATAAA
• BKK31220 (ΔrhaEW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTGATATTCCTCCAT,  downstream forward: _UP4_TAGATATATGCTATGATAAA

References

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