ykuP

168

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation

Locus: BSU_14170

Molecular weight: 20.11 kDa

Isoelectric point: 4.1

Protein length: 151 aa

Gene length: 456 bp

Function: electron transfer

Product: flavodoxin

Essential: no

Synonyms: ykuP

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Electron transport and ATP synthesis, Electron transport/ other

List of homologs in different organisms, belongs to COG0716 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, ResD regulon, NsrR regulon, Fur regulon, SigI regulon, Kre regulon

Gene

Coordinates: 1,488,413 → 1,488,868

The protein

Protein family

flavodoxin family (with YkuN, according to UniProt)

Domains

Flavodoxin-like domain (aa 4-144) (according to UniProt)

Cofactors

FMN

Structure

6FSG (PDB) (from B. cereus, 46% identity) PubMed
O34589 (AlphaFold)

Paralogous protein(s)

YkuN

Expression and Regulation

Operons

Genes: ykuN-ykuO-ykuP

Description: PubMed

Regulation

expressed under anaerobic conditions (ResD) PubMed
induced by iron starvation (second wave) (Fur) PubMed

Regulatory mechanism

ResD: activation, PubMed, in resD regulon
Kre: repression, in kre regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon
SigI: sigma factor, PubMed, in sigI regulon

Additional information

the ''YkuN-YkuO-YkuP operon is strongly unregulated in a kre'' mutant PubMed

Biological materials

Mutant

GP4732 ΔykuP::aphA3, available in Jörg Stülke's lab
MGNA-B339 (ykuP::erm), available at the NBRP B. subtilis, Japan
BKE14170 (ΔykuP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT,  downstream forward: _UP4_TGATCACTCACTGGGAACTG
BKK14170 (ΔykuP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT,  downstream forward: _UP4_TGATCACTCACTGGGAACTG

References

Loading