amhX

168

amidohydrolase

Locus: BSU_03010

Molecular weight: 42.37 kDa

Isoelectric point: 6.12

Protein length: 383 aa

Gene length: 1152 bp

Function: unknown

Product: amidohydrolase

Essential: no

Synonyms: amhX

Genomic Context

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Categories containing this gene/protein

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG1473 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CodY regulon

Gene

Coordinates: 324,038  325,189

The protein

Protein family

peptidase M20 family (according to UniProt)

Structure

1YSJ (PDB) (from Bacillus subtilis (32%) )
P54983 (AlphaFold)

Paralogous protein(s)

SndB

Expression and Regulation

Operons

Genes: amhX

Description: PubMed

Regulatory mechanism

CodY: repression, PubMed, in codY regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

BKE03010 (amhX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTAAGAAATACCTCCT,  downstream forward: _UP4_TAACGCAAAAAAAGCAGCCT
BKK03010 (amhX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTAAGAAATACCTCCT,  downstream forward: _UP4_TAACGCAAAAAAAGCAGCCT

References

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