carB

168

carbamoyl-P transferase (arginine) (subunit B)

Locus: BSU_11240

Molecular weight: 112.75 kDa

Isoelectric point: 4.71

Protein length: 1030 aa

Gene length: 3093 bp

Function: biosynthesis of arginine

Product: carbamoyl-P transferase (arginine) (subunit B)

Essential: no

E.C.: 6.3.5.5

Synonyms: carB

Genomic Context

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List of homologs in different organisms, belongs to COG0458 (Galperin et al., 2021)

Gene

Coordinates: 1,200,381 1,203,473

The protein

Catalyzed reaction/ biological activity

• 2 ATP + H₂O + bicarbonate + L-glutamine --> 2 ADP + carbamoyl-P + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• carB family (with PyrAB, according to UniProt)

Domains

• 2 ATP-grasp doamins (aa 133-327, aa 675-863) (according to UniProt)
• MGS-like domain (aa 925-1027) (according to UniProt)

Structure

• 1JDB (PDB) (from Escherichia coli, 47% identity, 64% similarity) PubMed
• P18185 (AlphaFold)

Paralogous protein(s)

• PyrAB

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: argC-argJ-argB-argD-carA-carB-argF

  Description: PubMed

  Regulation

  • repressed by arginine (AhrC) PubMed
  • induced in the absence of RnpM PubMed

  Regulatory mechanism

  • AhrC: repression, PubMed, in ahrC regulon
  • CodY: repression, PubMed, in codY regulon
  • RnpM: unknown, PubMed, in rnpM regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE11240 (carB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAATATGCTTGAAATACTGG, downstream forward: _UP4_CTCTATAAAAAGGAAGTGGC
• BKK11240 (carB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TAATATGCTTGAAATACTGG, downstream forward: _UP4_CTCTATAAAAAGGAAGTGGC
• GP3715 (carB::aphA3 trpC2) available in the Stülke lab

Expression vectors

• pGP3783: expression of Strep-carB by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

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