pheA

168

prephenate dehydratase

Locus: BSU_27900

Molecular weight: 31.75 kDa

Isoelectric point: 5.89

Protein length: 285 aa

Gene length: 858 bp

Function: biosynthesis of phenylalanine

Product: prephenate dehydratase

Essential: no

E.C.: 4.2.1.51

Synonyms: pheA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0077 (Galperin et al., 2021)

Gene

Coordinates: 2,851,283 → 2,852,140

The protein

Catalyzed reaction/ biological activity

• H⁺ + prephenate --> 3-phenylpyruvate + CO₂ + H₂O (according to UniProt)

Domains

• Prephenate dehydratase domain (aa 2-183) (according to UniProt)
• ACT domain (aa 204-281) (according to UniProt)

Structure

• 4LUB (PDB) (from Streptococcus mutans, 42% identity)
• P21203 (AlphaFold)

Effectors of protein activity

• subject to feedback inhibtion by phenylalanine PubMed

Expression and Regulation

Operons

• Genes: spo0B-obg-thrR-pheA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • expression is repressed by binding of RpoE to the A-rich sequence in the -35 region of the promoter PubMed

Biological materials

Mutant

• 1A617 ( pheA::erm), PubMed, available at BGSC
• BKE27900 (ΔpheA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACTTTCATGACGATTTTCT,  downstream forward: _UP4_TAAAAAAAGCCCACTAGAGG
• BKK27900 (ΔpheA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACTTTCATGACGATTTTCT,  downstream forward: _UP4_TAAAAAAAGCCCACTAGAGG

References

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