yukE

168

secreted protein of the WXG100 superfamily, homolog of virulence factor EsxA

Locus: BSU_31910

Molecular weight: 9.00 kDa

Isoelectric point: 7.91

Protein length: 97 aa

Gene length: 294 bp

Function: secretion and delivery of LXG domain toxins

Product: LXG domain toxin delivery factor

Essential: no

Synonyms: yukE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4842 (Galperin et al., 2021)

Gene

Coordinates: 3,276,141 3,276,434

The protein

Catalyzed reaction/ biological activity

• secretion and delivery of LXG domain toxins PubMed

Protein family

• WXG100 family (with YfjA, according to UniProt) (pfam06013) PubMed

Structure

• 3ZBH (PDB) (from Geobacillus thermodenitrificans, 59% identity)
• C0SP85 (AlphaFold)

Localization

• secreted, by the type VII protein secretion system YukD-EssB-YukB-YueB-YueC PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: yukE

  Description: PubMed

• Genes: yukE-yukD-essB-yukB-yueB-yueC-yueD

  Description: PubMed

  Regulation

  • expressed in the stationary phase PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in degU regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A628 (yukE::erm), available at the NBRP B. subtilis, Japan
• BKE31910 (yukE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCCTCATTACCTCCT, downstream forward: _UP4_TAACAGTATGAAAGGGAAGG
• BKK31910 (yukE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCCTCATTACCTCCT, downstream forward: _UP4_TAACAGTATGAAAGGGAAGG

References

Reviews

Loading

Original Publications

Loading