coaA

168

type I pantothenate/ pantetheine kinase, major enzyme

Locus: BSU_23760

Molecular weight: 36.49 kDa

Isoelectric point: 5.89

Protein length: 319 aa

Gene length: 960 bp

Function: biosynthesis of coenzyme A

Product: pantothenate kinase, pantetheine kinase

Essential: no

E.C.: 2.7.1.33

Synonyms: coaA, yqjS, panK

Genomic Context

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List of homologs in different organisms, belongs to COG1072 (Galperin et al., 2021)

Gene

Coordinates: 2,468,549 → 2,469,508

Phenotypes of a mutant

• a coaA coaX double mutant is not viable PubMed

The protein

Catalyzed reaction/ biological activity

• (R)-pantothenate + ATP --> (R)-4'-phosphopantothenate + ADP + H⁺ PubMed
• pantetheine + ATP --> phosphopantetheine + ADP + H⁺ PubMed

Protein family

• prokaryotic pantothenate kinase family (single member, according to UniProt)

Structure

• 1SQ5 (PDB) (from Escherichia coli, 49% identity, 67% similarity) PubMed
• P54556 (AlphaFold)

Expression and Regulation

Operons

• Genes: yqjP-yqjQ-dsdA-coaA-yqjT

  Description: PubMed

Biological materials

Mutant

• MGNA-C399 (yqjS::erm), available at the NBRP B. subtilis, Japan
• BKE23760 (ΔcoaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTTTACACCTGACTCT,  downstream forward: _UP4_GTGTTGGTAAGGAGGGTATG
• BKK23760 (ΔcoaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTTTACACCTGACTCT,  downstream forward: _UP4_GTGTTGGTAAGGAGGGTATG
• GP3355 (''coaA''::lox72), available in Jörg Stülke's lab
• GP3380 (coaA::neo trpC2), available in Jörg Stülke's lab PubMed

References

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