MotA

• Description: H+-coupled MotA-MotB flagellar stator
  
  

Gene name	motA
Synonyms	mot
Essential	no
Product	motility protein (flagellar motor rotation)
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: motA
Interactions involving this protein in SubtInteract: MotA
MW, pI	29 kDa, 5.01
Gene length, protein length	810 bp, 270 aa
Immediate neighbours	motB, clpE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU13690

Phenotypes of a mutant

• loss of swimming and swarming motility PubMed
• mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed

Database entries

• BsubCyc: BSU13690

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: motA family (according to Swiss-Prot)

• Paralogous protein(s): MotP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • the interaction with DgrA inhibits MotA activity PubMed

• Interactions:
  • MotA-MotB
  • DgrA-MotA PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13690

• Structure:

• UniProt: P28611

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: motA-motB PubMed

• Expression browser: motA PubMed

• Sigma factor: SigD PubMed
• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 100 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 613 PubMed

Biological materials

• Mutant:
  • 1A631 ( motA::erm), PubMed, available at BGSC
  • 1A923 ( motA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yuka Takahashi, Masahiro Ito
Mutational analysis of charged residues in the cytoplasmic loops of MotA and MotP in the Bacillus subtilis flagellar motor.
J Biochem: 2014, 156(4);211-20
[PubMed:24771657] [WorldCat.org] [DOI] (I p)

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

M Z Gilman, M J Chamberlin
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell: 1983, 35(1);285-93
[PubMed:6313226] [WorldCat.org] [DOI] (P p)