gerBC

168

lipoprotein, nutrient receptor, germination response to the combination of glucose, fructose, and KCl

Locus: BSU_35820

Molecular weight: 42.31 kDa

Isoelectric point: 9.06

Protein length: 374 aa

Gene length: 1125 bp

Function: germination

Product: part of the putative nutrient-gated GerBA-GerBB-GerBC ion channel

Essential: no

Synonyms: gerBC

Genomic Context

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List of homologs in different organisms, belongs to COG5903 (Galperin et al., 2021)

Gene

Coordinates: 3,691,372  3,692,496

The protein

Catalyzed reaction/ biological activity

• the GerBA-GerBB-GerBC ion channel likely releases ions from the spore core in response to asparagine, glucose, fructose and K⁺ to trigger Germination, this requires the gerK locus PubMed

Protein family

• GerABKC lipoprotein family (according to UniProt)

Domains

• has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide PubMed

Structure

• 3N54 (PDB) PubMed
• P39571 (AlphaFold)

Paralogous protein(s)

• GerAC

Localization

• outer surface of the inner spore membrane PubMed

Additional information

• 700 molecules are present per spore PubMed

Expression and Regulation

Operons

• Genes: gerBA-gerBB-gerBC

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

  Additional information

  • 700 molecules are present per spore PubMed

Biological materials

Mutant

• BKE35820 (gerBC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACGGAGAATTTTGATGCTG,  downstream forward: _UP4_TAAGCAATCAAAAGGGTGCG
• BKK35820 (gerBC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACGGAGAATTTTGATGCTG,  downstream forward: _UP4_TAAGCAATCAAAAGGGTGCG

References

Reviews

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Original Publications

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