yugT

168

similar to exo-alpha-1,4-glucosidase

Locus: BSU_31290

Molecular weight: 63.82 kDa

Isoelectric point: 4.97

Protein length: 554 aa

Gene length: 1665 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 3.2.1.10

Synonyms: yugT

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of other polymeric carbohydrates

Lifestyles, Sporulation, Sporulation proteins, Newly identified sporulation proteins (based on transcription profiling)

List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

This gene is a member of the following regulons

Spx regulon

Gene

Coordinates: 3,214,372  3,216,036

The protein

Catalyzed reaction/ biological activity

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose (according to UniProt)

Protein family

glycosyl hydrolase 13 family (according to UniProt)

Structure

2ZE0 (PDB) (from Geobacillus sp., 64% identity), PubMed
O05242 (AlphaFold)

Paralogous protein(s)

TreA, YcdG, MalL

Expression and Regulation

Operons

Genes: yugT

Description: PubMed

Regulatory mechanism

Spx: activation, PubMed, in spx regulon

Biological materials

Mutant

MGNA-B547 (yugT::erm), available at the NBRP B. subtilis, Japan
BKE31290 (yugT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAGCCACTCCTTCCCA,  downstream forward: _UP4_TAAACTCCAGCCTTAGACTG
BKK31290 (yugT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAGCCACTCCTTCCCA,  downstream forward: _UP4_TAAACTCCAGCCTTAGACTG

References

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