racA

168

cell division protein

Locus: BSU_37030

Molecular weight: 20.93 kDa

Isoelectric point: 9.51

Protein length: 184 aa

Gene length: 555 bp

Product: cell division protein

Essential: no

Synonyms: racA, ywkC

Genomic Context

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List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

Gene

Coordinates: 3,798,789  3,799,343

The protein

Protein family

• racA family (single member, according to UniProt)

Domains

• HTH merR-type domain (aa 75-156) (according to UniProt)

Structure

• 5I44 (PDB) (RacA-DN complex) PubMed
• 5I41 (PDB) (DNA-binding domain) PubMed
• P45870 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ywkD-racA

  Description: PubMed

• Genes: racA

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon

  Sigma factors

  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• MGNA-B655 (ywkC::erm), available at the NBRP B. subtilis, Japan
• BKE37030 (racA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCCCATACCTCCTTT,  downstream forward: _UP4_TAAAGCAAAAAGCTCCCTTA
• BKK37030 (racA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCCCATACCTCCTTT,  downstream forward: _UP4_TAAAGCAAAAAGCTCCCTTA

References

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