racA

168

cell division protein

locus: BSU_37030

Molecular weight: 20.93 kDa

pI: 9.51

Protein length: 184 aa

Gene length: 555 bp

function: chromosome segregation in sporulating cells

product: cell division protein

essential: no

synonyms: racA, ywkC

List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

Gene

Coordinates: 3,798,789  3,799,343

The protein

Catalyzed reaction/ biological activity

• chromosome tethering by interacting with both centromere-like elements in the DNA and with [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA] [pubmed|31239381]
• contributes to efficient transport of DNA by the chromosome segregation motor [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE] [pubmed|31239381]

Protein family

• racA family (single member, according to UniProt)

[wiki|Domains]

• [wiki|HTH merR-type domain] (aa 75-156) (according to UniProt)

Structure

• [PDB|5I44] (RacA-DN complex) [Pubmed|27085804]
• [PDB|5I41] (DNA-binding domain) [Pubmed|27085804]
• [AF|P45870]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|81CCDC33856F2F8BC59BCC7FED34015340D21C48|ywkD]-[gene|09E9194E82DF199527F414DB0EC15547A71AD25E|racA]

  description: [Pubmed|22383849]

• genes: [gene|09E9194E82DF199527F414DB0EC15547A71AD25E|racA]

  description: [pubmed|22383849]

  regulation

  • expressed under conditions that trigger [wiki|sporulation ]([wiki|Spo0A]) [Pubmed|12493822,14651647,15687200]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|12493822,14651647,15687200], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|12493822], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]

Biological materials

Mutant

• MGNA-B655 (ywkC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1654 NBRP B. subtilis, Japan]
• BKE37030 ([gene|09E9194E82DF199527F414DB0EC15547A71AD25E|racA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCCCATACCTCCTTT,  downstream forward: _UP4_TAAAGCAAAAAGCTCCCTTA
• BKK37030 ([gene|09E9194E82DF199527F414DB0EC15547A71AD25E|racA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATCCCATACCTCCTTT,  downstream forward: _UP4_TAAAGCAAAAAGCTCCCTTA

References

• 27085804,15780934,19478798,12493822,12950914,21630458,14651647,15687200,12493822,23264578,31239381