murP

168

N-acetyl muramic acid permease of the phosphotransferase system, EIIBC of the PTS

Locus: BSU_01680

Molecular weight: 47.44 kDa

Isoelectric point: 8.5

Protein length: 455 aa

Gene length: 1368 bp

Function: N-acetyl muramic acid uptake and phosphorylation

Product: N-acetyl muramic acid PTS permease, EIIBC component

Essential: no

E.C.: 2.7.1.69

Synonyms: murP, ybbF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

Gene

Coordinates: 189,790  191,157

The protein

Catalyzed reaction/ biological activity

• transport and concomitant phosphorylation of N-acetyl muramic acid

Protein family

• PTS permease, sucrose family PubMed

Domains

• PTS EIIB domain type-1 (aa 8-90) (according to UniProt)
• PTS EIIC domain type-1 (aa 116-455) (according to UniProt)

Structure

• Q797S1 (AlphaFold)

Paralogous protein(s)

• BglP, SacX, SacP, TreP

Expression and Regulation

Operons

• Genes: murQ-murR-murP-amiE-nagZ-namZ

  Description: PubMed

  Regulation

  • expressed in late exponential and early stationary phase PubMed

  Regulatory mechanism

  • MurR: repression, PubMed, in murR regulon

Biological materials

Mutant

• MGNA-B950 (ybbF::erm), available at the NBRP B. subtilis, Japan
• BKE01680 (murP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC,  downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG
• BKK01680 (murP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC,  downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG

References

Reviews

Loading

Original Publications

Loading