abnA

168

endo-1,5-alpha-L-arabinosidase

Locus: BSU_28810

Molecular weight: 34.13 kDa

Isoelectric point: 8.62

Protein length: 323 aa

Gene length: 972 bp

Function: arabinan degradation

Product: endo-1,5-alpha-L-arabinosidase

Essential: no

E.C.: 3.2.1.99

Synonyms: abnA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3507 (Galperin et al., 2021)

Gene

Coordinates: 2,949,053  2,950,024

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans (according to UniProt)

Protein family

• glycosyl hydrolase 43 family (according to UniProt)

Structure

• 1UV4 (PDB)
• P94522 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: abnA

  Description: PubMed

  Regulation

  • repressed in the absence of arabinose (AraR) PubMed

  Regulatory mechanism

  • AraR: repression, PubMed, in araR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE28810 (abnA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTTGTTCAACCTCCTT,  downstream forward: _UP4_TGAATTAAAAAGCCGGGCTC
• BKK28810 (abnA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTTGTTCAACCTCCTT,  downstream forward: _UP4_TGAATTAAAAAGCCGGGCTC

References

Loading