ComER

• Description: non-essential gene for competence
  
  

Gene name	comER
Synonyms	comD, comED
Essential	no
Product	putative pyrroline-5'-carboxylate reductase
Function	genetic compentence
Gene expression levels in SubtiExpress: comER
MW, pI	30 kDa, 6.183
Gene length, protein length	819 bp, 273 aa
Immediate neighbours	comEA, yqeM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins, sporulation proteins

The gene

Basic information

• Locus tag: BSU25600

Database entries

• BsubCyc: BSU25600

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU25600

• Structure:

• UniProt: P39696

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comER PubMed

• Expression browser: comER PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for comER PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582] [WorldCat.org] [DOI] (P p)

J Hahn, G Inamine, Y Kozlov, D Dubnau
Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.
Mol Microbiol: 1993, 10(1);99-111
[PubMed:7968523] [WorldCat.org] [DOI] (P p)