mdxF

168

maltodextrin ABC transporter, permease

Locus: BSU_34600

Molecular weight: 48.37 kDa

Isoelectric point: 8.78

Protein length: 435 aa

Gene length: 1308 bp

Function: maltodextrin utilization

Product: maltodextrin ABC transporter, permease

Essential: no

Synonyms: mdxF, yvdH

Genomic Context

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List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,553,206 → 3,554,513

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• MalFG subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 195-422) (according to UniProt)

Structure

• 2R6G (PDB) (from E. coli, C-terminal domain, aa 173-427, 36% identity) PubMed
• O06990 (AlphaFold)

Paralogous protein(s)

• GanP

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: mdxR-yvdF-mdxE-mdxF-mdxG-yvdJ-yvdK-malL-pgcM

  Description: PubMed

  Regulation

  • induced in the presence of starch, β-cyclomaltodextrin, or maltose PubMed

  Regulatory mechanism

  • MdxR: activation, PubMed, in mdxR regulon

Biological materials

Mutant

• MGNA-B626 (yvdH::erm), available at the NBRP B. subtilis, Japan
• BKE34600 (ΔmdxF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCGCCCTCTATTC,  downstream forward: _UP4_TTTGGCAATGAGGAGGTTAT
• BKK34600 (ΔmdxF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCGCCCTCTATTC,  downstream forward: _UP4_TTTGGCAATGAGGAGGTTAT

References

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