lutB

lutB
168

lactate catabolic enzyme

Locus
BSU_34040
Molecular weight
53.31 kDa
Isoelectric point
5.85
Protein length
Gene length
Function
lactate utilization
Product
lactate oxidase
Essential
no
Synonyms
lutB, yvfW

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1139 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,493,519  3,494,958
Phenotypes of a mutant
no growth with lactate as the single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
oxidation of lactate to pyruvate PubMed
Protein family
LutB/YkgF family (single member, according to UniProt)
2 4Fe-4S ferredoxin-type domains (aa 304-334, aa 353-382) (according to UniProt)
[4Fe-4S] cluster
Structure
Modification
phosphorylated on Arg-33 PubMed
Expression and Regulation
Operons
Description
Regulation
induction by lactate PubMed
Regulatory mechanism
SinR: repression, PubMed, in sinR regulon
LutR: repression, in lutR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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lutAlutC

2025-02-13 14:07:03

ghost

87

7db2bc789fad5a99442f7b9ff99a43f2b42b4a12

59CEB598935063C72EA3D1BE77C54FD6CCE3442E

Other regulations
FsrA: translation repression, PubMed
FbpB: translation inhibition, acts as RNA chaperone, increases interaction between FsrA and LutA-LutB-LutC RNAs
Biological materials
Mutant
MGNA-A498 (yvfW::erm), available at the NBRP B. subtilis, Japan
BKE34040 (lutB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGATCCTTTCCCCCTCTG,  downstream forward: _UP4_GAACGCACGAAGGAGGAGCA
BKK34040 (lutB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGATCCTTTCCCCCTCTG,  downstream forward: _UP4_GAACGCACGAAGGAGGAGCA
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
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Time of last update: 2025-02-24 10:49:54

Author of last update: Jstuelk