yvcT

168

similar to hydroxypyruvate reductase

Locus: BSU_34680

Molecular weight: 36.46 kDa

Isoelectric point: 5.09

Protein length: 325 aa

Gene length: 978 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 1.1.1.215

Synonyms: yvcT

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Groups of genes, Proteins of unknown function

List of homologs in different organisms, belongs to COG1052 (Galperin et al., 2021)

Gene

Coordinates: 3,562,566 → 3,563,543

The protein

Catalyzed reaction/ biological activity

D-gluconate + NADP⁺ --> 2-dehydro-D-gluconate + H⁺ + NADPH (according to UniProt)

Protein family

D-isomer specific 2-hydroxyacid dehydrogenase family (with YoaD and SerA, according to UniProt)

Structure

5AOV (PDB) (from Pyrococcus furiosus, 46% identity) PubMed
O32264 (AlphaFold)

Additional information

The gene is annotated in KEGG as gluconate 2-dehydrogenase EC 1.1.1.215. The gene is marked “similar to glycerate dehydrogenase” in MetaCyc and marked as probable EC 1.1.1.215 in Swiss-Prot. No literature/experimental evidence supporting the annotation is available.  PubMed

Expression and Regulation

Operons

Genes: yvcT

Description: PubMed

Biological materials

Mutant

MGNA-B633 (yvcT::erm), available at the NBRP B. subtilis, Japan
BKE34680 (ΔyvcT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC,  downstream forward: _UP4_TAAATCAAAAATCCGGTCTG
BKK34680 (ΔyvcT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC,  downstream forward: _UP4_TAAATCAAAAATCCGGTCTG

References

Research papers

Loading