Difference between revisions of "YvcK"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 96 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 96 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 246 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 246 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 555 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 282 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 459 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:'''
 
* '''Mutant:'''
  

Revision as of 14:22, 17 April 2014

  • Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1

Gene name yvcK
Synonyms
Essential no
Product unknown
Function localization of PBP1
Gene expression levels in SubtiExpress: yvcK
MW, pI 34 kDa, 5.408
Gene length, protein length 951 bp, 317 aa
Immediate neighbours yvcL, yvcJ
Sequences Protein DNA DNA_with_flanks
Genetic context
YvcK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YvcK expression.png















Categories containing this gene/protein

carbon core metabolism, cell shape

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU34760

Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0052 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 96 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 246 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 555 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 282 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 459 PubMed

Biological materials

  • Mutant:
  • Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Your additional remarks

References

Reviews

PubMed

Original publications