ytrE

168

ABC transporter (ATP-binding protein)

Locus: BSU_30420

Molecular weight: 25.31 kDa

Isoelectric point: 5.98

Protein length: 231 aa

Gene length: 696 bp

Function: unknown

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms: ytrE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1136 (Galperin et al., 2021)

Gene

Coordinates: 3,115,279  3,115,974

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 4-231) (according to UniProt)

Structure

• 1L2T (PDB) (from Methanocaldococcus jannaschii, 44% identity) PubMed
• O34392 (AlphaFold)

Paralogous protein(s)

• YxdL, FtsE, BceA, YknY, YvrO

Localization

• cell membrane (via YtrC-YtrD) PubMed

Expression and Regulation

Operons

• Genes: ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF

  Description: PubMed

  Regulation

  • expressed early in the stationary phase PubMed

  Regulatory mechanism

  • YtrA: repression, PubMed, in ytrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP3191 (ΔytrE), available in Jörg Stülke's lab PubMed
• GP2646 Δ(ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF)::ermC, available in Jörg Stülke's lab PubMed
• MGNA-B537 (ytrE::erm), available at the NBRP B. subtilis, Japan
• BKE30420 (ytrE::erm  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_ATCAATCATATGTTCTCTCT,  downstream forward: _UP4_GGTGTGCTAAAAGGAGGAAT
• BKK30420 (ytrE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAATCATATGTTCTCTCT,  downstream forward: _UP4_GGTGTGCTAAAAGGAGGAAT

References

Loading