yraA

168

general stress protein, degradation of damaged thiol-containing proteins, glyoxalase III-like enzyme

Locus: BSU_27020

Molecular weight: 18.37 kDa

Isoelectric point: 4.73

Protein length: 169 aa

Gene length: 510 bp

Function: detoxification of methylglyoxal

Product: glyoxalase III-like enzyme

Essential: no

Synonyms: yraA

Genomic Context

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List of homologs in different organisms, belongs to COG0693 (Galperin et al., 2021)

Gene

Coordinates: 2,757,492  2,758,001

The protein

Catalyzed reaction/ biological activity

• methylglyoxal - D-lactate PubMed

Protein family

• peptidase C56 family (according to UniProt)

Domains

• PfpI endopeptidase domain (aa 3-169) (according to UniProt)

Structure

• 4Y0N (PDB) (from Staphylococcus aureus, 57% identity) PubMed
• O06006 (AlphaFold)

Paralogous protein(s)

• YfkM

Expression and Regulation

Operons

• Genes: yrzP-adhA-yraA

  Description: PubMed

• Genes: yraA

  Description: NULL

  Regulation

  • ''adhA'': induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde (AdhR) PubMed

  Regulatory mechanism

  • AdhR: activation, PubMed, in adhR regulon

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon
  • SigM: sigma factor, PubMed, in sigM regulon

• Genes: adhA-yraA

  Description: PubMed

  Regulation

  • ''adhA'': induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde (AdhR) PubMed

  Regulatory mechanism

  • AdhR: activation, PubMed, in adhR regulon

Biological materials

Mutant

• MGNA-A239 (yraA::erm), available at the NBRP B. subtilis, Japan
• BKE27020 (yraA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAAACCCTCCTAATA,  downstream forward: _UP4_TAACAAGAAGGCACAGACTG
• BKK27020 (yraA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAAACCCTCCTAATA,  downstream forward: _UP4_TAACAAGAAGGCACAGACTG

References

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