yqfO

168

similar to GTP cyclohydrolase

Locus: BSU_25170

Molecular weight: 40.76 kDa

Isoelectric point: 5.39

Protein length: 373 aa

Gene length: 1122 bp

Function: unknown

Product: similar to GTP cyclohydrolase

Essential: no

Synonyms: yqfO

Genomic Context

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List of homologs in different organisms, belongs to COG3323 (Galperin et al., 2021)

Gene

Coordinates: 2,597,502 → 2,598,623

The protein

Protein family

• GTP cyclohydrolase I type 2/NIF3 family (single member, according to UniProt)

Structure

• 2GX8 (PDB) (from B. cereus, 61% identity) PubMed
• P54472 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: trmK-yqfO

  Description

  • PubMed

Biological materials

Mutant

• GP4365 (trpC2 ΔyqfO::neo), available in Jörg Stülke's lab
• MGNA-C490 (yqfO::erm), available at the NBRP B. subtilis, Japan
• BKE25170 (ΔyqfO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATGATCTGCTGCCCATTTA,  downstream forward: _UP4_TAAAAAAACTGAGGCTTTTA
• BKK25170 (ΔyqfO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATGATCTGCTGCCCATTTA,  downstream forward: _UP4_TAAAAAAACTGAGGCTTTTA

References

Research papers

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