engA

168

GTPase essential for ribosome 50S subunit assembly

Locus: BSU_22840

Molecular weight: 48.61 kDa

Isoelectric point: 5.25

Protein length: 436 aa

Gene length: 1311 bp

Function: ribosome assembly

Product: GTPase

Essential: yes

Synonyms: yphC

Genomic Context

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List of homologs in different organisms, belongs to COG1160 (Galperin et al., 2021)

Gene

Coordinates: 2,390,206 → 2,391,516

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds and hydrolyzes GTP and readily exchanges GDP for GTP
• participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit PubMed

Protein family

• TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily (according to UniProt)

Domains

• 2 EngA-type G domains (aa 4-167, aa 176-351) (according to UniProt)
• KH-like domain (aa 352-436) (according to UniProt)

Cofactors

• Mg²⁺ PubMed

Structure

• 2HJG (PDB) (complex with GDP) PubMed
• 5M7H (PDB) (complex with GMPPNP and PO4)
• P50743 (AlphaFold)

Paralogous protein(s)

• YyaF, Era, YsxC, RbgA, ThdF, Obg, YqeH, YnbA

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: engA-gpsA

  Description: PubMed

• Genes: yphA-seaA-ypzH-engA-gpsA

  Description: PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• MGNA-A402 (yphC::erm), available at the NBRP B. subtilis, Japan

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP846,  available in Jörg Stülke's lab

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

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Original Publications

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