pepF

168

oligoendopeptidase, inhibits sporulation upon overexpression

Locus: BSU_11540

Molecular weight: 69.97 kDa

Isoelectric point: 5.13

Protein length: 670 aa

Gene length: 2013 bp

Function: protein degradation

Product: oligoendopeptidase

Essential: no

Synonyms: pepF, yjbG

Genomic Context

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Categories containing this gene/protein

Information processing, Regulation of gene expression, phosphorelay

Lifestyles, Sporulation, phosphorelay

List of homologs in different organisms, belongs to COG1164 (Galperin et al., 2021)

Gene

Coordinates: 1,231,083  1,233,095

The protein

Protein family

peptidase M3B family (single member, according to UniProt)

Structure

2QR4 (PDB) (from Enterococcus faecium, 52% identity)
O31605 (AlphaFold)

Paralogous protein(s)

YusY/1

Expression and Regulation

Operons

Genes: pepF

Description: PubMed

Genes: coiA-pepF

Description: PubMed

Biological materials

Mutant

MGNA-B154 (yjbG::erm), available at the NBRP B. subtilis, Japan
1A823 ( pepF::erm), PubMed, available at BGSC
BKE11540 (pepF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGCCTGCCCACCACCTT,  downstream forward: _UP4_TAAAAAAGTAAGCCTGTGCG
BKK11540 (pepF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGCCTGCCCACCACCTT,  downstream forward: _UP4_TAAAAAAGTAAGCCTGTGCG

References

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