yhfE

168

similar to aminopeptidase

locus: BSU_10200

Molecular weight: 38.58 kDa

pI: 5.88

Protein length: 346 aa

Gene length: 1041 bp

function: unknown

product: unknown

essential: no

synonyms: yhfE

List of homologs in different organisms, belongs to COG1363 (Galperin et al., 2021)

Gene

Coordinates: 1,095,063 → 1,096,103

The protein

Protein family

• Peptidase M42 family (with [protein|D6F5F7C1C699C674FA7AC09A020E8E2F571E1AA9|ysdC] and [protein|16585F4118F3D2732D6780D351B7AA5F6DDCCEB8|ytoP], according to UniProt)

Structure

• [PDB|2GRE] (from ''Bacillus Cereus'', 71% identity)
• [AF|O07603]

Expression and Regulation

Operons

• genes: [gene|EDD5ACB89661D3B6D77057863EF345EBF84F49B2|yhfE]-[gene|A666F376947A675E04550338C3177FF8AFF44DF4|yhfF]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A710 (yhfE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/710 NBRP B. subtilis, Japan]
• BKE10200 (Δ[gene|EDD5ACB89661D3B6D77057863EF345EBF84F49B2|yhfE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGGGCATTCCTCCTTTT,  downstream forward: _UP4_CCAATGGTATAAGGGGGAGG
• BKK10200 (Δ[gene|EDD5ACB89661D3B6D77057863EF345EBF84F49B2|yhfE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGGGCATTCCTCCTTTT,  downstream forward: _UP4_CCAATGGTATAAGGGGGAGG