pdxK

168

pyridoxine, pyridoxal, and pyridoxamine kinase

Locus: BSU_38020

Molecular weight: 28.87 kDa

Isoelectric point: 4.92

Protein length: 271 aa

Gene length: 816 bp

Function: biosynthesis of pyridoxal phosphate

Product: pyridoxine, pyridoxal, and pyridoxamine kinase

Essential: no

E.C.: 2.7.4.7

Synonyms: pdxK, ywdB, ipa-52r, thiD

Genomic Context

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List of homologs in different organisms, belongs to COG0351 (Galperin et al., 2021)

Gene

Coordinates: 3,900,963 → 3,901,778

The protein

Catalyzed reaction/ biological activity

• ATP + pyridoxal --> ADP + H⁺ + pyridoxal 5'-phosphate (according to UniProt)

Protein family

• ThiD family (with ThiD, according to UniProt)

Structure

• 2I5B (PDB) PubMed
• P39610 (AlphaFold)

Paralogous protein(s)

• ThiD

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: pdxK

  Description: PubMed

Biological materials

Mutant

• BKE38020 (ΔpdxK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCTTGAACCTCCATCA,  downstream forward: _UP4_TAAAAAAAGGGGCTGCCTTA
• BKK38020 (ΔpdxK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCTTGAACCTCCATCA,  downstream forward: _UP4_TAAAAAAAGGGGCTGCCTTA

References

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