thiC

168

4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase, biosynthesis of the pyrimidine moiety of thiamine

Locus: BSU_08790

Molecular weight: 65.74 kDa

Isoelectric point: 5.26

Protein length: 590 aa

Gene length: 1773 bp

Function: biosynthesis of thiamine

Product: 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase

Essential: no

Synonyms: thiC, thiA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0422 (Galperin et al., 2021)

Gene

Coordinates: 955,895  957,667

The protein

Catalyzed reaction/ biological activity

• 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + S-adenosyl-L-methionine --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H⁺ + L-methionine (according to UniProt)

Protein family

• thiC family (single member, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 3EPN (PDB) (from Caulobacter crescentus, 65% identity) PubMed
• P45740 (AlphaFold)

Modification

• phosphorylated on Arg-556 PubMed
• phosphorylated on Ser-565, Ser-586 and Tyr-589 PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: thiC

  Description: PubMed

  Regulation

  • repressed by thiamine (Thi-box) PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, in Thi-box regulon

Biological materials

Mutant

• 1A603 ( thiC::erm), PubMed, available at BGSC
• BKE08790 (thiC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT,  downstream forward: _UP4_TAAAAAAAATGAAGATGGAG
• BKK08790 (thiC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT,  downstream forward: _UP4_TAAAAAAAATGAAGATGGAG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

Loading

Original Publications

Loading