spoVG

168

RNA-binding regulatory protein, negative effector of asymetric septation at the onset of sporulation

Locus: BSU_00490

Molecular weight: 10.75 kDa

Isoelectric point: 5.11

Protein length: 97 aa

Gene length: 294 bp

Function: cell division, control of sporulation initiation

Product: negative effector of asymetric septation

Essential: no

Synonyms: spoVG

Genomic Context

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List of homologs in different organisms, belongs to COG2088 (Galperin et al., 2021)

Gene

Coordinates: 55,866 → 56,159

The protein

Protein family

• SpoVG family (single member, according to UniProt)

Structure

• 2IA9 (PDB)
• P28015 (AlphaFold)

Modification

• phosphorylation on (Ser-66 OR Ser-67 OR Thr-68) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: spoVG

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • SinR: repression, PubMed, in sinR regulon
  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• MGNA-A082 (spoVG::erm), available at the NBRP B. subtilis, Japan
• GP2109 (''spoVG::tet'', available in Jörg Stülke's lab)
• BKE00490 (ΔspoVG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTAGTTCACCACCTTT,  downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
• BKK00490 (ΔspoVG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTAGTTCACCACCTTT,  downstream forward: _UP4_TAAAAAATAACCAAAAAGCA

Expression vectors

• pGP2163: expression of SpoVG-Strep by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
• pGP2310: expression of SpoVG-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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