serC

168

3-phosphoserine aminotransferase

Locus: BSU_10020

Molecular weight: 39.98 kDa

Isoelectric point: 5.52

Protein length: 359 aa

Gene length: 1080 bp

Function: biosynthesis of serine

Product: 3-phosphoserine aminotransferase

Essential: no

E.C.: 2.6.1.52

Synonyms: serC, yhaF, glyB

Genomic Context

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List of homologs in different organisms, belongs to COG1932 (Galperin et al., 2021)

Gene

Coordinates: 1,075,289 1,076,368

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + O-phospho-L-serine --> 3-phosphooxypyruvate + L-glutamate (according to UniProt)
• 2-oxoglutarate + 4-(phosphooxy)-L-threonine --> (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate (according to UniProt)

Protein family

• Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP

Structure

• 1W23 (PDB) (from Bacillus alcalophilus, 59% identity) PubMed
• P80862 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: serC-trpP

  Description: PubMed

Biological materials

Mutant

• MGNA-A685 (serC::erm), available at the NBRP B. subtilis, Japan
• 1A621 ( serC::erm), PubMed, available at BGSC
• BKE10020 (serC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTATGATCTCTCCCTGTT, downstream forward: _UP4_TAAAAAGTCTGGCTATGCAA
• BKK10020 (serC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTATGATCTCTCCCTGTT, downstream forward: _UP4_TAAAAAGTCTGGCTATGCAA
• BP1274 (serC::ermC trp+) available in Jörg Stülke's & Fabian Commichau's lab

References

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