rsbV

168

antagonist of RsbW, anti-anti-SigB

Locus: BSU_04710

Molecular weight: 11.80 kDa

Isoelectric point: 4.7

Protein length: 109 aa

Gene length: 330 bp

Function: control of SigB activity

Product: anti-anti-SigB

Essential: no

Synonyms: rsbV

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1366 (Galperin et al., 2021)

Gene

Coordinates: 522,088  522,417

The protein

Protein family

• anti-sigma-factor antagonist family (with SpoIIAA, according to UniProt)

Domains

• STAS domain (aa 3-109) (according to UniProt)

Structure

• 1VC1 (PDB) (homolog from Thermotoga maritima)
• P17903 (AlphaFold)

Modification

• phosphorylation on Ser-52 AND Ser-56 by RsbW PubMed, PubMed

Expression and Regulation

Operons

• Genes: rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX

  Description: PubMed

  Regulation

  • ''rsbV:'' induced by stress (SigB) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: rsbV-rsbW-sigB-rsbX

  Description: PubMed

  Regulation

  • ''rsbV:'' induced by stress (SigB) PubMed

Biological materials

Mutant

• BKE04710 (rsbV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCGTATCACCTCAAATT,  downstream forward: _UP4_GCAAAGTCAGAAGGTGGAGT
• BKK04710 (rsbV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCGTATCACCTCAAATT,  downstream forward: _UP4_GCAAAGTCAGAAGGTGGAGT

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Reviews

Loading

Original Publications

Loading