pyrC

168

dihydroorotase

Locus: BSU_15500

Molecular weight: 46.45 kDa

Isoelectric point: 5.43

Protein length: 428 aa

Gene length: 1287 bp

Function: pyrimidine biosynthesis

Product: dihydroorotase

Essential: no

E.C.: 3.5.2.3

Synonyms: pyrC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)

Gene

Coordinates: 1,621,374 → 1,622,660

The protein

Catalyzed reaction/ biological activity

• (S)-dihydroorotate + H₂O --> H⁺ + N-carbamoyl-L-aspartate (according to UniProt)

Protein family

• Metallo-dependent hydrolases superfamily (according to UniProt)

Structure

• 3GRI (PDB) (from Staphylococcus aureus subsp. aureus mw2, 60% identity, 72% similarity)
• P25995 (AlphaFold)

Paralogous protein(s)

• PucH

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE

  Description: PubMed

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

  Regulatory mechanism

  • PyrR: termination, via RNA switch, in pyrR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE15500 (ΔpyrC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC,  downstream forward: _UP4_TATGAAGAGGGGAGACTTGT
• BKK15500 (ΔpyrC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC,  downstream forward: _UP4_TATGAAGAGGGGAGACTTGT

References

Loading