purM

168

phosphoribosylaminoimidazole synthetase

Locus: BSU_06500

Molecular weight: 36.90 kDa

Isoelectric point: 4.59

Protein length: 346 aa

Gene length: 1041 bp

Function: purine biosynthesis

Product: phosphoribosylaminoimidazole synthetase

Essential: no

E.C.: 6.3.3.1

Synonyms: purM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0150 (Galperin et al., 2021)

Gene

Coordinates: 706,973  708,013

The protein

Catalyzed reaction/ biological activity

• 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + ATP --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ADP + H⁺ + phosphate (according to UniProt)

Protein family

• AIR synthase family (single member, according to UniProt)

Structure

• 2BTU (PDB) (from Bacillus anthracis, 64% identity, 78% similarity)
• P12043 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD

  Description: PubMed

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

  Regulatory mechanism

  • G-box: RNA switch, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• 1A601 ( purM::erm), PubMed, available at BGSC
• BKE06500 (purM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTATTCACTCCTTTAA,  downstream forward: _UP4_ACATTCGGCGGTGCGGCACT
• BKK06500 (purM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTATTCACTCCTTTAA,  downstream forward: _UP4_ACATTCGGCGGTGCGGCACT

References

Loading