purM

purM
168

phosphoribosylaminoimidazole synthetase

Locus
BSU_06500
Molecular weight
36.90 kDa
Isoelectric point
4.59
Protein length
Gene length
Function
purine biosynthesis
Product
phosphoribosylaminoimidazole synthetase
Essential
no
E.C.
6.3.3.1
Synonyms
purM

Genomic Context

List of homologs in different organisms, belongs to COG0150 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, G-box, PurR regulon

Gene
Coordinates
706,973  708,013
The protein
Catalyzed reaction/ biological activity
2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + ATP --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ADP + H+ + phosphate (according to UniProt)
Protein family
AIR synthase family (single member, according to UniProt)
Structure
2BTU (PDB) (from Bacillus anthracis, 64% identity, 78% similarity)
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Description
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purEpurD

2025-07-26 17:54:31

Jstuelk

263

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
1A601 ( purM::erm), PubMed, available at BGSC
BKE06500 (purM::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCCTATTCACTCCTTTAA,  downstream forward: _UP4_ACATTCGGCGGTGCGGCACT
BKK06500 (purM::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCCTATTCACTCCTTTAA,  downstream forward: _UP4_ACATTCGGCGGTGCGGCACT
References
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Page visits: 5497

Time of last update: 2025-07-26 23:37:14

Author of last update: Melvin.boenninger