prs

168

phosphoribosylpyrophosphate synthetase, universally conserved protein

Locus: BSU_00510

Molecular weight: 34.71 kDa

Isoelectric point: 5.89

Protein length: 317 aa

Gene length: 954 bp

Function: phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)

Product: phosphoribosylpyrophosphate synthetase

Essential: yes

E.C.: 2.7.6.1

Synonyms: prs

Genomic Context

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List of homologs in different organisms, belongs to COG0462 (Galperin et al., 2021)

Gene

Coordinates: 57,745  58,698

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + D-ribose 5-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + AMP + H⁺ (according to UniProt)

Protein family

• Ribose-phosphate pyrophosphokinase family (single member, according to UniProt)

Structure

• 1IBS (PDB)
• 1DKR (PDB)
• 1DKU (PDB)
• P14193 (AlphaFold)

Effectors of protein activity

• subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) PubMed

Localization

• cytoplasm (according to UniProt)

Additional information

• universally conserved protein

Expression and Regulation

Operons

• Genes: gcaD-prs-ctc

  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Expression vectors

• N-terminal Strep-tag, expression in E. coli, in pGP172: pGP2541, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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