pgm

pgm
168

phosphoglycerate mutase, glycolytic / gluconeogenic enzyme

Locus
BSU_33910
Molecular weight
56.14 kDa
Isoelectric point
5.21
Protein length
Gene length
Function
enzyme in glycolysis / gluconeogenesis
Product
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Essential
Yes
E.C.
5.4.2.1
Synonyms
pgm, gpmI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0696 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,477,877 3,479,412
Phenotypes of a mutant
Essential PubMed
The protein
Catalyzed reaction/ biological activity
(2R)-2-phosphoglycerate --> 3-phosphoglycerate (according to UniProt)
Protein family
BPG-independent phosphoglycerate mutase family (single member, according to UniProt)
Mn2+
Structure
1EJJ (PDB) (Geobacillus stearothermophilus, complex with 3-phosphoglycerate), 1EQJ (PDB) (Geobacillus stearothermophilus, complex with 2-phosphoglycerate), Geobacillus stearothermophilus, complex with 2-phosphoglycerate NCBI, Geobacillus stearothermophilus, complex with 3-phosphoglycerate NCBI
Modification
phosphorylation on Ser-62 PubMed, PubMed
Effectors of protein activity
Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione PubMed
2,3-Diphosphoglycerate has NO role on this enzyme regulation PubMed
Kinetic information
Reversible Michaelis-Menten PubMed
Cytoplasm (Homogeneous) PubMed
Additional information
extensive information on the structure and enzymatic properties of Pgm can be found at Proteopedia
Expression and Regulation
Operons
Description
Regulation
expression induced by glycolytic intermediates (CggR) CggR PubMed
the mRNA is processed between cggR and gapA by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
CggR: repression, PubMed, in cggR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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cggReno

2025-07-25 14:18:33

Jstuelk

201

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

Genes
Description
Open in new tab

pgkeno

2025-07-24 14:17:33

Jstuelk

155

c718c56bfee7b3945ef7060e9c50614ecf6b1cd7

D821B77CDDBA7B2EDE01B5B3E2E0A964498A261D

Biological materials
Mutant
GP593 (pgm::cat), available in Jrg Stlke's lab, PubMed
GP598 (pgm::erm), available in Jrg Stlke's lab, PubMed
GP698 (pgm-eno::cat), available in Jrg Stlke's lab, PubMed
Expression vectors
pGP1425 (expression of pgm in B. subtilis, in pBQ200), available in Jrg Stlke's lab
pGP1500 (expression of pgm and eno in B. subtilis, in pBQ200), available in Jrg Stlke's lab
pGP1101 (N-terminal His-tag, in pWH844), available in Jrg Stlke's lab
pGP396 (Pgm-S62A, N-terminal His-tag, in pWH844), available in Jrg Stlke's lab
pGP92 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jrg Stlke's lab, PubMed
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab, PubMed
Labs working on this gene/protein
Jrg Stlke, University of Gttingen, Germany Homepage
Mark J. Jedrzejas, Research Center Oakland, CA, USA Homepage
References
Commichau FM, Pietack N, Stülke J Essential genes in Bacillus subtilis: a re-evaluation after ten years. Molecular bioSystems. 2013 Jun; 9(6):1068-75. doi:10.1039/c3mb25595f. PMID:23420519
Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics. 2007 Oct; 7(19):3509-26. . PMID:17726680
Jannière L, Canceill D, Suski C, Kanga S, Dalmais B, Lestini R, Monnier AF, Chapuis J, Bolotin A, Titok M, Le Chatelier E, Ehrlich SD Genetic evidence for a link between glycolysis and DNA replication. PloS one. 2007 May 16; 2(5):e447. . PMID:17505547
Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Molecular & cellular proteomics : MCP. 2007 Apr; 6(4):697-707. . PMID:17218307
Nukui M, Mello LV, Littlejohn JE, Setlow B, Setlow P, Kim K, Leighton T, Jedrzejas MJ Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: a crucial enzyme for spores and growing cells of Bacillus species. Biophysical journal. 2007 Feb 01; 92(3):977-88. . PMID:17085493
Nukui M, Mello LV, Littlejohn JE, Setlow B, Setlow P, Kim K, Leighton T, Jedrzejas MJ Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: a crucial enzyme for spores and growing cells of Bacillus species. Biophysical journal. 2007 Feb 01; 92(3):977-88. . PMID:17085493
Meile JC, Wu LJ, Ehrlich SD, Errington J, Noirot P Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory. Proteomics. 2006 Apr; 6(7):2135-46. . PMID:16479537
Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metabolic engineering. 2003 Apr; 5(2):133-49. . PMID:12850135
Rigden DJ, Lamani E, Mello LV, Littlejohn JE, Jedrzejas MJ Insights into the catalytic mechanism of cofactor-independent phosphoglycerate mutase from X-ray crystallography, simulated dynamics and molecular modeling. Journal of molecular biology. 2003 May 09; 328(4):909-20. . PMID:12729763
Rigden DJ, Mello LV, Setlow P, Jedrzejas MJ Structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus with broad specificity phosphatase activity. Journal of molecular biology. 2002 Feb 01; 315(5):1129-43. . PMID:11827481
Jedrzejas MJ, Setlow P Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate. Chemical reviews. 2001 Mar; 101(3):607-18. . PMID:11712498
Jedrzejas MJ, Setlow P Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate. Chemical reviews. 2001 Mar; 101(3):607-18. . PMID:11712498
Rigden DJ, Bagyan I, Lamani E, Setlow P, Jedrzejas MJ A cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus is actually a broad specificity phosphatase. Protein science : a publication of the Protein Society. 2001 Sep; 10(9):1835-46. . PMID:11514674
Ludwig H, Homuth G, Schmalisch M, Dyka FM, Hecker M, Stülke J Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Molecular microbiology. 2001 Jul; 41(2):409-22. . PMID:11489127
Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus. Crystal structure of the complex with 2-phosphoglycerate. The Journal of biological chemistry. 2000 Jul 28; 275(30):23146-53. . PMID:10764795
Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus. Crystal structure of the complex with 2-phosphoglycerate. The Journal of biological chemistry. 2000 Jul 28; 275(30):23146-53. . PMID:10764795
Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus. The EMBO journal. 2000 Apr 03; 19(7):1419-31. . PMID:10747010
Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus. The EMBO journal. 2000 Apr 03; 19(7):1419-31. . PMID:10747010
Chander M, Setlow P, Lamani E, Jedrzejas MJ Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus. Journal of structural biology. 1999 Jun 15; 126(2):156-65. . PMID:10388626
Chander M, Setlow P, Lamani E, Jedrzejas MJ Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus. Journal of structural biology. 1999 Jun 15; 126(2):156-65. . PMID:10388626
Chander M, Setlow B, Setlow P The enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires Mn2+ and is pH sensitive. Canadian journal of microbiology. 1998 Aug; 44(8):759-67. . PMID:9830105
Magill NG, Cowan AE, Leyva-Vazquez MA, Brown M, Koppel DE, Setlow P Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species. Journal of bacteriology. 1996 Apr; 178(8):2204-10. . PMID:8636019
Kuhn NJ, Setlow B, Setlow P Manganese(II) activation of 3-phosphoglycerate mutase of Bacillus megaterium: pH-sensitive interconversion of active and inactive forms. Archives of biochemistry and biophysics. 1993 Nov 01; 306(2):342-9. . PMID:8215434
Leyva-Vazquez MA, Setlow P Cloning and nucleotide sequences of the genes encoding triose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis. Journal of bacteriology. 1994 Jul; 176(13):3903-10. . PMID:8021172
Watabe K, Freese E Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis. Journal of bacteriology. 1979 Feb; 137(2):773-8. . PMID:33963

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Time of last update: 2025-07-29 22:04:44

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