pgm

168

[metabolite|phosphoglycerate] mutase, glycolytic / gluconeogenic enzyme

locus: BSU_33910

Molecular weight: 56.14 kDa

pI: 5.21

Protein length: 511 aa

Gene length: 1536 bp

function: enzyme in glycolysis / gluconeogenesis

product: 2,3-bisphosphoglycerate-independent [metabolite|phosphoglycerate] mutase

essential: Yes

ec: 5.4.2.1

synonyms: pgm, gpmI

List of homologs in different organisms, belongs to COG0696 (Galperin et al., 2021)

Gene

Coordinates: 3,477,877 3,479,412

Phenotypes of a mutant

• Essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• (2R)-[metabolite|2-phosphoglycerate] --> 3-[metabolite|phosphoglycerate] (according to UniProt)

Protein family

• BPG-independent [metabolite|phosphoglycerate] mutase family (single member, according to UniProt)

[wiki|Cofactors]

• Mn2+

Structure

• [PDB|1EJJ] (Geobacillus stearothermophilus, complex with 3-[metabolite|phosphoglycerate]), [PDB|1EQJ] (Geobacillus stearothermophilus, complex with [metabolite|2-phosphoglycerate]), ''Geobacillus stearothermophilus'', complex with [metabolite|2-phosphoglycerate] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=16359 NCBI], ''Geobacillus stearothermophilus'', complex with 3-[metabolite|phosphoglycerate] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=15578 NCBI]
• [AF|P39773]

Modification

• phosphorylation on Ser-62 [Pubmed|17218307], [Pubmed|17726680]

Effectors of protein activity

• Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione [Pubmed|33963]
• 2,3-Diphosphoglycerate has NO role on this enzyme regulation [Pubmed|33963]

Kinetic information

• Reversible Michaelis-Menten [Pubmed|33963]

[wiki|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Additional information

• extensive information on the structure and enzymatic properties of Pgm can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglycerate_Mutase Proteopedia]

Expression and Regulation

Operons

• genes: [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]

  description: [Pubmed|11489127]

  regulation

  • expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
  • the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

  regulatory mechanism

  • [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]

  description: [Pubmed|11489127]

Biological materials

Mutant

• GP593 (''pgm''::''cat''), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
• GP598 (''pgm''::''erm''), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
• GP698 (''pgm''-''eno''::cat), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]

Expression vectors

• pGP1425 (expression of ''pgm'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jrg Stlke]'s lab
• pGP1500 (expression of ''pgm'' and ''eno'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jrg Stlke]'s lab
• pGP1101 (N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jrg Stlke]'s lab
• pGP396 (Pgm-S62A, N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jrg Stlke]'s lab
• pGP92 (N-terminal Strep-tag, for [wiki|SPINE], expression in B. subtilis, in [wiki|pGP380]), available in [wiki|Jrg Stlke]'s lab, [pubmed|19193632]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab, [pubmed|19193632]

labs

• [wiki|Jrg Stlke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
• [wiki|Mark J. Jedrzejas], Research Center Oakland, CA, USA [http://www.chori.org/Principal_Investigators/Jedrzejas_Mark_J/jedrzejas_research.html Homepage]

References

• 11514674,17085493,10764795,8215434,10747010,11712498,9830105,10388626,8636019,11827481,16479537,12850135,17726680,17505547,8021172,11489127,10388626,10747010,10764795,11712498,12729763,17085493,17218307,33963,23420519