pgm
168
phosphoglycerate mutase, glycolytic / gluconeogenic enzyme
Locus
BSU_33910
Molecular weight
56.14 kDa
Isoelectric point
5.21
Function
enzyme in glycolysis / gluconeogenesis
Product
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Essential
Yes
E.C.
5.4.2.1
Synonyms
pgm, gpmI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0696 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
The protein
Catalyzed reaction/ biological activity
(2R)-2-phosphoglycerate --> 3-phosphoglycerate (according to UniProt)
Protein family
BPG-independent phosphoglycerate mutase family (single member, according to UniProt)
Mn2+
Structure
1EJJ (PDB) (Geobacillus stearothermophilus, complex with 3-phosphoglycerate), 1EQJ (PDB) (Geobacillus stearothermophilus, complex with 2-phosphoglycerate), Geobacillus stearothermophilus, complex with 2-phosphoglycerate NCBI, Geobacillus stearothermophilus, complex with 3-phosphoglycerate NCBI
Modification
Effectors of protein activity
Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione PubMed
2,3-Diphosphoglycerate has NO role on this enzyme regulation PubMed
Kinetic information
Reversible Michaelis-Menten PubMed
Cytoplasm (Homogeneous) PubMed
Additional information
extensive information on the structure and enzymatic properties of Pgm can be found at Proteopedia
Expression and Regulation
Operons
Biological materials
Mutant
Expression vectors
Two-hybrid system
Labs working on this gene/protein
Mark J. Jedrzejas, Research Center Oakland, CA, USA Homepage
References
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Molecular bioSystems. 2013 Jun; 9(6):1068-75. doi:10.1039/c3mb25595f. PMID:23420519
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Proteomics. 2007 Oct; 7(19):3509-26. . PMID:17726680
Genetic evidence for a link between glycolysis and DNA replication.
PloS one. 2007 May 16; 2(5):e447. . PMID:17505547
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Molecular & cellular proteomics : MCP. 2007 Apr; 6(4):697-707. . PMID:17218307
Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: a crucial enzyme for spores and growing cells of Bacillus species.
Biophysical journal. 2007 Feb 01; 92(3):977-88. . PMID:17085493
Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: a crucial enzyme for spores and growing cells of Bacillus species.
Biophysical journal. 2007 Feb 01; 92(3):977-88. . PMID:17085493
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics. 2006 Apr; 6(7):2135-46. . PMID:16479537
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metabolic engineering. 2003 Apr; 5(2):133-49. . PMID:12850135
Insights into the catalytic mechanism of cofactor-independent phosphoglycerate mutase from X-ray crystallography, simulated dynamics and molecular modeling.
Journal of molecular biology. 2003 May 09; 328(4):909-20. . PMID:12729763
Structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus with broad specificity phosphatase activity.
Journal of molecular biology. 2002 Feb 01; 315(5):1129-43. . PMID:11827481
Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate.
Chemical reviews. 2001 Mar; 101(3):607-18. . PMID:11712498
Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate.
Chemical reviews. 2001 Mar; 101(3):607-18. . PMID:11712498
A cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus is actually a broad specificity phosphatase.
Protein science : a publication of the Protein Society. 2001 Sep; 10(9):1835-46. . PMID:11514674
Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon.
Molecular microbiology. 2001 Jul; 41(2):409-22. . PMID:11489127
Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus. Crystal structure of the complex with 2-phosphoglycerate.
The Journal of biological chemistry. 2000 Jul 28; 275(30):23146-53. . PMID:10764795
Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus. Crystal structure of the complex with 2-phosphoglycerate.
The Journal of biological chemistry. 2000 Jul 28; 275(30):23146-53. . PMID:10764795
Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus.
The EMBO journal. 2000 Apr 03; 19(7):1419-31. . PMID:10747010
Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus.
The EMBO journal. 2000 Apr 03; 19(7):1419-31. . PMID:10747010
Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus.
Journal of structural biology. 1999 Jun 15; 126(2):156-65. . PMID:10388626
Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus.
Journal of structural biology. 1999 Jun 15; 126(2):156-65. . PMID:10388626
The enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires Mn2+ and is pH sensitive.
Canadian journal of microbiology. 1998 Aug; 44(8):759-67. . PMID:9830105
Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species.
Journal of bacteriology. 1996 Apr; 178(8):2204-10. . PMID:8636019
Manganese(II) activation of 3-phosphoglycerate mutase of Bacillus megaterium: pH-sensitive interconversion of active and inactive forms.
Archives of biochemistry and biophysics. 1993 Nov 01; 306(2):342-9. . PMID:8215434
Cloning and nucleotide sequences of the genes encoding triose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis.
Journal of bacteriology. 1994 Jul; 176(13):3903-10. . PMID:8021172
Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis.
Journal of bacteriology. 1979 Feb; 137(2):773-8. . PMID:33963
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Time of last update: 2025-07-29 22:04:44
Author of last update: Jstuelk