patA

168

N-acetyl-L,L-diaminopimelate aminotransferase

Locus: BSU_14000

Molecular weight: 43.29 kDa

Isoelectric point: 6.01

Protein length: 393 aa

Gene length: 1182 bp

Function: biosynthesis of lysine and peptidoglycan

Product: aminotransferase

Essential: yes

E.C.: 2.6.1.-

Synonyms: patA, uat, dapX

Genomic Context

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List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

Gene

Coordinates: 1,471,857  1,473,038

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• N-acetyl-2-amino-6-ketopimelate - N-acetyl-L,L-2,6-diaminopimelate PubMed
• 2-oxoglutarate + N-acetyl-LL-2,6-diaminoheptanedioate --> L-2-acetamido-6-oxoheptanedioate + L-glutamate (according to UniProt)

Protein family

• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP

Structure

• 1GDE (PDB) (from Pyrococcus sp., 42% identity) PubMed
• P16524 (AlphaFold)

Paralogous protein(s)

• AlaT, AspB

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: patA

  Description: PubMed

• Genes: ykzT-patA

  Description: PubMed

Biological materials

Mutant

• BKE14000 (patA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATTCATCACCTTAACA,  downstream forward: _UP4_AACAACGGCGTTTAAGCCGT

References

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