pabB

168

para-aminobenzoate synthase (subunit A)

Locus: BSU_00740

Molecular weight: 53.09 kDa

Isoelectric point: 5.23

Protein length: 470 aa

Gene length: 1413 bp

Function: biosynthesis of folate

Product: para-aminobenzoate synthase (subunit A)

Essential: no

E.C.: 2.6.1.85

Synonyms: pabB, pab

Genomic Context

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List of homologs in different organisms, belongs to COG0147 (Galperin et al., 2021)

Gene

Coordinates: 82,864 → 84,276

The protein

Catalyzed reaction/ biological activity

• Chorismate + L-glutamine --> 4-amino-4-deoxychorismate + L-glutamate (according to UniProt)

Protein family

• Anthranilate synthase component I family (with TrpE, according to UniProt)

Structure

• 7PI1 (PDB)
• 5CWA (PDB) (from Mycobacterium tuberculosis, 39% identity) PubMed
• P28820 (AlphaFold)

Expression and Regulation

Operons

• Genes: pabB-pabA-pabC-sul-folB-folK-yazB-dusB1-lysS

  Description: PubMed

  Regulation

  • translation repressed by tryptophan (MtrB) PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE00740 (ΔpabB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTATCATTCCTCTAT,  downstream forward: _UP4_AAAATTAGATGAGGTGAGCG
• BKK00740 (ΔpabB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTATCATTCCTCTAT,  downstream forward: _UP4_AAAATTAGATGAGGTGAGCG

References

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