nin

168

inhibitor of the DNA degrading activity of NucA

Locus: BSU_03420

Molecular weight: 14.86 kDa

Isoelectric point: 4.92

Protein length: 132 aa

Gene length: 399 bp

Function: genetic transformation, DNA uptake

Product: inhibitor of the DNA degrading activity of NucA

Essential: no

Synonyms: nin, comJ

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 371,729  372,127

The protein

Structure

• 4MQD (PDB)
• P12669 (AlphaFold)

Modification

• phosphorylated on Arg-96 PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: nucA-nin

  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

Biological materials

Mutant

• BKE03420 (nin::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTGCTGTTCCTCCTCT,  downstream forward: _UP4_TAATGAAAAACCCCTGAGAG
• BKK03420 (nin::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTGCTGTTCCTCCTCT,  downstream forward: _UP4_TAATGAAAAACCCCTGAGAG

References

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