moeA

168

molybdopterin molybdenumtransferase

Locus: BSU_14280

Molecular weight: 46.46 kDa

Isoelectric point: 5.61

Protein length: 430 aa

Gene length: 1293 bp

Function: nitrate respiration

Product: molybdopterin molybdenumtransferase

Essential: no

E.C.: 2.10.1.1

Synonyms: moeA

Genomic Context

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List of homologs in different organisms, belongs to COG0303 (Galperin et al., 2021)

Gene

Coordinates: 1,497,192  1,498,484

The protein

Catalyzed reaction/ biological activity

• adenylyl-molybdopterin + H⁺ + molybdate --> AMP + H₂O + Mo-molybdopterin (according to UniProt)

Protein family

• MoeA family (single member, according to UniProt)

Structure

• 1T3E (PDB) (from rat, 35% identity)
• O31703 (AlphaFold)

Expression and Regulation

Operons

• Genes: mobA-moeB-moeA-mobB-moaE-moaD

  Description: PubMed

Biological materials

Mutant

• BKE14280 (moeA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAGCATTCTGACCCCTCCTG,  downstream forward: _UP4_ATCAGAGAAGAGAATGGAAG
• BKK14280 (moeA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAGCATTCTGACCCCTCCTG,  downstream forward: _UP4_ATCAGAGAAGAGAATGGAAG

References

Reviews

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Original Publications

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