mntA

168

manganese ABC transporter (binding protein, lipoprotein)

Locus: BSU_30770

Molecular weight: 33.26 kDa

Isoelectric point: 6.16

Protein length: 306 aa

Gene length: 921 bp

Function: manganese uptake

Product: manganese ABC transporter (binding protein)

Essential: no

Synonyms: mntA, ytgA

Genomic Context

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List of homologs in different organisms, belongs to COG0803 (Galperin et al., 2021)

Gene

Coordinates: 3,145,038  3,145,958

Phenotypes of a mutant

• essential according to Kobayashi et al., but a mutant has been constructed and studied by Que and Helmann, 2000
• knockdown of expression results in loss of ICEBs1 conjugation (both in donor cells) PubMed

The protein

Protein family

• bacterial solute-binding protein 9 family (with ZnuA, according to UniProt)

Structure

• 1TOA (PDB) (zinc-binding protein from Treponema pallidum, 46% identity) PubMed
• O34385 (AlphaFold)

Paralogous protein(s)

• ZnuA

Localization

• cell membrane (according to Swiss-Prot),  extracellular lipoprotein (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: mntA-mntB-mntC-mntD

  Description: PubMed

  Regulation

  • repressed at high Mn(II) concentrations (MntR) PubMed

  Regulatory mechanism

  • MntR: repression, PubMed, in mntR regulon

  Additional information

  • A ncRNA is predicted between 'mntA' and 'menC' PubMed

Biological materials

Mutant

• MGNA-A285 (ytgA::erm), available at the NBRP B. subtilis, Japan
• BKE30770 (mntA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG,  downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA
• BKK30770 (mntA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG,  downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

Reviews

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Original Publications

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