mlpA

168

metallopeptidase, involved in regulation of protease gene expression

Locus: BSU_16710

Molecular weight: 45.80 kDa

Isoelectric point: 5.28

Protein length: 409 aa

Gene length: 1230 bp

Function: control of proteolytic activity

Product: specific processing protease

Essential: no

Synonyms: mlpA, ymxG

Genomic Context

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List of homologs in different organisms, belongs to COG0612 (Galperin et al., 2021)

Gene

Coordinates: 1,742,617  1,743,846

Phenotypes of a mutant

• fivefold increased levels of proteolytic activity in their growth medium PubMed

The protein

Protein family

• Peptidase M16 family (according to UniProt)

Structure

• 3HDI (PDB) (from B. halodurans, 64% identity) PubMed
• Q04805 (AlphaFold)

Expression and Regulation

Operons

• Genes: mlpA

  Description: PubMed

Biological materials

Mutant

• MGNA-A057 (ymxG::erm), available at the NBRP B. subtilis, Japan
• deletion mutant,  available in van Dijl lab
• BKE16710 (mlpA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTTGTTCCTCCTATTC,  downstream forward: _UP4_TAAAAAGGAAAGCCTGCCCC
• BKK16710 (mlpA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTTGTTCCTCCTATTC,  downstream forward: _UP4_TAAAAAGGAAAGCCTGCCCC

References

Reviews

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Original Publications

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Labs working on this gene/protein

• Jan Maarten van Dijl, Groningen, Netherlands