metK

168

S-adenosylmethionine synthetase

Locus: BSU_30550

Molecular weight: 43.88 kDa

Isoelectric point: 5.03

Protein length: 400 aa

Gene length: 1203 bp

Function: S-adenosylmethionine synthesis

Product: S-adenosylmethionine synthetase

Essential: yes

E.C.: 2.5.1.6

Synonyms: metK, metE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0192 (Galperin et al., 2021)

Gene

Coordinates: 3,127,825 3,129,027

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-methionine --> diphosphate + phosphate + S-adenosyl-L-methionine (according to UniProt)

Protein family

• AdoMet synthase family (single member, according to UniProt)

Structure

• 1FUG (PDB) (from Escherichia coli, 63% identity, 75% similarity) PubMed
• P54419 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: metK-asnB-alaP

  Description: PubMed

  Regulation

  • constitutive

• Genes: metK

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed

  Regulatory mechanism

  • S-box: RNA switch, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

Biological materials

LacZ fusion

• pGP432 (in pAC7), available in Jrg Stlke's lab

References

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