hxlA

168

3-hexulose-6-phosphate synthase

Locus: BSU_03460

Molecular weight: 22.43 kDa

Isoelectric point: 4.56

Protein length: 210 aa

Gene length: 633 bp

Function: ribulose monophosphate pathway for formaldehyde fixation

Product: 3-hexulose-6-phosphate synthase

Essential: no

E.C.: 4.1.2.43

Synonyms: hxlA, yckG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0269 (Galperin et al., 2021)

Gene

Coordinates: 375,166 → 375,798

The protein

Catalyzed reaction/ biological activity

• D-ribulose 5-phosphate + formaldehyde --> D-arabino-hex-3-ulose 6-phosphate (according to UniProt)

Protein family

• HPS/KGPDC family (single member, according to UniProt)

Structure

• 3F4W (PDB) (from Salmonella typhimurium, 44% identity)
• P42405 (AlphaFold)

Expression and Regulation

Operons

• Genes: hxlA-hxlB

  Description: PubMed

  Regulation

  • induction by formaldehyde (HxlR) PubMed

  Regulatory mechanism

  • HxlR: activation, PubMed, in hxlR regulon

Biological materials

Mutant

• MGNA-C057 (yckG::erm), available at the NBRP B. subtilis, Japan
• BKE03460 (ΔhxlA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTGATCCACTCCCTT,  downstream forward: _UP4_CTGATTGTCCAAGGATAACT
• BKK03460 (ΔhxlA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTGATCCACTCCCTT,  downstream forward: _UP4_CTGATTGTCCAAGGATAACT

References

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