hutU

168

urocanase

locus: BSU_39360

Molecular weight: 60.43 kDa

pI: 5.66

Protein length: 552 aa

Gene length: 1659 bp

function: histidine utilization

product: urocanase

essential: no

ec: 4.2.1.49

synonyms: hutU

List of homologs in different organisms, belongs to COG2987 (Galperin et al., 2021)

Gene

Coordinates: 4,043,574 → 4,045,232

The protein

Catalyzed reaction/ biological activity

• 4-imidazolone-5-propanoate --> H₂O + trans-urocanate (according to UniProt)

Protein family

• urocanase family (single member, according to UniProt)

Structure

• [PDB|2FKN]
• [AF|P25503]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]-[gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH]-[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]-[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]-[gene|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG]-[gene|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM]

  description: [Pubmed|10746760]

  regulation

  • induced by histidine ([protein|search|HutP]) [Pubmed|8071225]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8071225], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|8682780], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]: antitermination, at a protein-dependent [wiki|RNA switch] located between [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP] and [gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH] [Pubmed|8071225], in [regulon|protein:BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8071225], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE39360 (Δ[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGTCATATGTTCATCCCCCT,  downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA
• BKK39360 (Δ[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGTCATATGTTCATCCCCCT,  downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA

References

Reviews

• 22933560

Original Publications

• 10746760,8071225,8682780,10217782,21821766