hutU

168

urocanase

Locus: BSU_39360

Molecular weight: 60.43 kDa

Isoelectric point: 5.66

Protein length: 552 aa

Gene length: 1659 bp

Function: histidine utilization

Product: urocanase

Essential: no

E.C.: 4.2.1.49

Synonyms: hutU

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2987 (Galperin et al., 2021)

Gene

Coordinates: 4,043,574 → 4,045,232

The protein

Catalyzed reaction/ biological activity

• 4-imidazolone-5-propanoate --> H₂O + trans-urocanate (according to UniProt)

Protein family

• urocanase family (single member, according to UniProt)

Structure

• 2FKN (PDB)
• P25503 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hutP-hutH-hutU-hutI-hutG-hutM

  Description: PubMed

  Regulation

  • induced by histidine (HutP) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • CodY: repression, PubMed, in codY regulon
  • HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in hutP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE39360 (ΔhutU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTCATATGTTCATCCCCCT,  downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA
• BKK39360 (ΔhutU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTCATATGTTCATCCCCCT,  downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA

References

Reviews

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Original Publications

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