hprK

168

HPr kinase/ phosphorylase

Locus: BSU_35000

Molecular weight: 34.55 kDa

Isoelectric point: 4.91

Protein length: 310 aa

Gene length: 933 bp

Function: carbon catabolite repression,phosphorylation of HPr and Crh proteins at Ser46

Product: HPr kinase/ phosphorylase

Essential: no

Synonyms: hprK, ptsK, yvoB

Genomic Context

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List of homologs in different organisms, belongs to COG1493 (Galperin et al., 2021)

Gene

Coordinates: 3,594,242  3,595,174

Phenotypes of a mutant

• no carbon catabolite repression PubMed
• delay in spore germination PubMed

The protein

Catalyzed reaction/ biological activity

• [HPr protein]-L-serine + ATP --> [HPr protein]-O-phospho-L-serine + ADP + H⁺ (according to UniProt)
• [HPr protein]-O-phospho-L-serine + H⁺ + phosphate --> [HPr protein]-L-serine + diphosphate (according to UniProt)

Protein family

• HPrK/P family (single member, according to UniProt)

Structure

• 1KNX (PDB) (from Mycoplasma pneumoniae) PubMed
• 1KKM (PDB) (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P)
• 1KKL (PDB) (complex of Lactobacillus casei HprK with B. subtilis HPr)
• O34483 (AlphaFold)

Expression and Regulation

Operons

• Genes: hprK-lgt-yvoD-yvoE-yvoF

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A328 (hprK::erm), available at the NBRP B. subtilis, Japan
• GP202 (ΔhprK::spc) PubMed, available in Jörg Stülke's lab
• GP858 (ΔhprK::aphA3) PubMed, available in Jörg Stülke's lab
• GP82 (ΔhprK::cat) PubMed, available in Jörg Stülke's lab
• BKE35000 (ΔhprK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGATATGTTCCTCCTGTT,  downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
• BKK35000 (ΔhprK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGATATGTTCCTCCTGTT,  downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP642, available in Jörg Stülke's lab
• for expression/ purification of mutant HprK-G158A from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP650, available in Jörg Stülke's lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP205, available in Jörg Stülke's lab PubMed
• for expression, purification of the N-terminal domain in E. coli with N-terminal His-tag, in pWH844: pGP218, available in Jörg Stülke's lab

LacZ fusion

• pGP201 (in pAC5, PubMed) available in Jörg Stülke's lab
• pGP202 (in pAC6, PubMed) available in Jörg Stülke's lab

Antibody

• available in Jörg Stülke's lab PubMed

Labs working on this gene/protein

• Jörg Stülke, University of Göttingen, Germany Homepage
• Anne Galinier, University of Marseille, France

References

Reviews

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Structural Analysis of HPrK

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General Analysis, Physiology

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Enzymatic Properties, Mutation Analysis

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HprK as a Target For Antimicrobial Compounds

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