guaC

168

GMP reductase

Locus: BSU_32130

Molecular weight: 35.66 kDa

Isoelectric point: 6.08

Protein length: 326 aa

Gene length: 981 bp

Function: purine salvage and interconversion

Product: GMP reductase

Essential: no

E.C.: 1.7.1.7

Synonyms: guaC, yumD

Genomic Context

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List of homologs in different organisms, belongs to COG0516 (Galperin et al., 2021)

Gene

Coordinates: 3,303,042  3,304,022

The protein

Catalyzed reaction/ biological activity

• Inosine 5'-phosphate + NH₃ + NADP⁺ --> guanosine 5'-phosphate + NADPH (according to UniProt)

Protein family

• IMPDH/GMPR family (together with GuaB) (according to UniProt)

Structure

• 1YPF (PDB) (from Bacillus anthracis, 84% identity, 91% similarity)
• O05269 (AlphaFold)

Expression and Regulation

Operons

• Genes: guaC

  Description: PubMed

  Regulation

  • activated during growth in the presence of branched chain amino acids (CodY) PubMed

  Regulatory mechanism

  • CodY: activation, PubMed, in codY regulon
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3 to 20 min) PubMed

Biological materials

Mutant

• MGNA-B573 (yumD::erm), available at the NBRP B. subtilis, Japan
• BKE32130 (guaC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA,  downstream forward: _UP4_TAATCATAAAAAAACGCCAA
• BKK32130 (guaC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA,  downstream forward: _UP4_TAATCATAAAAAAACGCCAA

References

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