gsaB

168

glutamate-1-semialdehyde aminotransferase, required for Efp modification

Locus: BSU_08710

Molecular weight: 46.04 kDa

Isoelectric point: 5.64

Protein length: 429 aa

Gene length: 1290 bp

Function: biosynthesis of heme, modification of Efp

Product: glutamate-1-semialdehyde aminotransferase

Essential: no

E.C.: 5.4.3.8

Synonyms: gsaB

Genomic Context

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List of homologs in different organisms, belongs to COG0001 (Galperin et al., 2021)

Gene

Coordinates: 942,449  943,738

Phenotypes of a mutant

• inactivation suppresses the Swarming defect of a ymfI mutant PubMed
• defective in Swarming motility PubMed

The protein

Catalyzed reaction/ biological activity

• (S)-4-amino-5-oxopentanoate --> 5-aminolevulinate (according to UniProt)

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• pyridoxal phosphate (attached to Lys-267) (according to UniProt)

Structure

• 3BS8 (PDB) (HemL) PubMed
• P71084 (AlphaFold)

Modification

• pyridoxal phosphate attached to Lys-267 (according to UniProt)

Paralogous protein(s)

• HemL

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: gsaB

  Description: PubMed

Biological materials

Mutant

• BKE08710 (gsaB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAATAAAAACCTCCTAT,  downstream forward: _UP4_TAGAATCAAATACCTTGAAC
• BKK08710 (gsaB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAATAAAAACCTCCTAT,  downstream forward: _UP4_TAGAATCAAATACCTTGAAC

References

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