gcvPB

168

glycine decarboxylase (subunit 2)

Locus: BSU_24550

Molecular weight: 54.26 kDa

Isoelectric point: 5.28

Protein length: 488 aa

Gene length: 1467 bp

Function: glycine utilization

Product: glycine decarboxylase (subunit 2)

Essential: no

E.C.: 1.4.4.2

Synonyms: gcvPB, yqhK, gcvP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1003 (Galperin et al., 2021)

Gene

Coordinates: 2,545,410  2,546,876

The protein

Catalyzed reaction/ biological activity

• glycine + H⁺ + N⁶-lipoyl-L-lysyl-[glycine-cleavage complex H protein] --> (R)-N⁶-(S⁸-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO₂ (according to UniProt)

Protein family

• GcvP family (with GcvPA, according to UniProt)

Cofactors

• pyridoxal phosphate (according to UniProt)

Structure

• 1WYT (PDB) (the GcvPA-GcvPB complex from Thermus thermophilus, 56% identity) PubMed
• P54377 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: gcvT-gcvPA-gcvPB

  Description: PubMed

  Regulation

  • induced by glycine PubMed
  • the Gly-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • Gly-box: RNA switch, PubMed, in Gly-box

Biological materials

Mutant

• MGNA-C470 (yqhK::erm), available at the NBRP B. subtilis, Japan
• BKE24550 (gcvPB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAAAAATAAGTGCCTGGT,  downstream forward: _UP4_TAAATAAAAACAGCTGTCTA
• BKK24550 (gcvPB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAAAAATAAGTGCCTGGT,  downstream forward: _UP4_TAAATAAAAACAGCTGTCTA

References

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