fabI

168

enoyl-acyl carrier protein reductase

Locus: BSU_11720

Molecular weight: 27.72 kDa

Isoelectric point: 5.61

Protein length: 258 aa

Gene length: 777 bp

Function: fatty acid biosynthesis

Product: enoyl-acyl carrier protein reductase

Essential: no

E.C.: 1.3.1.9

Synonyms: fabI, yjbW

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0623 (Galperin et al., 2021)

Gene

Coordinates: 1,247,788 1,248,564

The protein

Catalyzed reaction/ biological activity

• 2,3-saturated acyl-[ACP] + NAD⁺ --> (2E)-enoyl-[ACP] + H⁺ + NADH (according to UniProt)

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Structure

• 3OIF (PDB) (complex with NAD and inhibitor triclosan) PubMed
• P54616 (AlphaFold)

Effectors of protein activity

• inhibited by triclosan PubMed

Paralogous protein(s)

• FabL, one of the two proteins has to be present for viability PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: fabI

  Description: PubMed

  Regulation

  • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed

  Regulatory mechanism

  • FapR: repression, PubMed, in fapR regulon

  Sigma factors

  • SigI: sigma factor, PubMed, in sigI regulon

Biological materials

Mutant

• MGNA-B168 (yjbW::erm), available at the NBRP B. subtilis, Japan
• BKE11720 (fabI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
• BKK11720 (fabI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG

References

Reviews

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Original Publications

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