etfB

168

electron transfer flavoprotein (beta subunit)

Locus: BSU_28530

Molecular weight: 28.37 kDa

Isoelectric point: 4.19

Protein length: 257 aa

Gene length: 774 bp

Function: fatty acid degradation

Product: electron transfer flavoprotein (beta subunit)

Essential: no

Synonyms: etfB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2086 (Galperin et al., 2021)

Gene

Coordinates: 2,916,378  2,917,151

The protein

Protein family

• ETF beta-subunit/fixA family (single member, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 1EFP (PDB) (from Paracoccus denitrificans, 37% identity) PubMed
• P94550 (AlphaFold)

Expression and Regulation

Operons

• Genes: lcfA-fadR-fadB-etfB-etfA

  Description: PubMed

  Regulation

  • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fadR-fadB-etfB-etfA

  Description: PubMed

  Regulation

  • induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• 1A855 ( etfB::cat), PubMed, available at BGSC
• BKE28530 (''etfB::erm'', available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
• BKE28530 (etfB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGATTCATGATCATATCCC,  downstream forward: _UP4_TAAAACTTGGACATTCAAAG
• BKK28530 (etfB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGATTCATGATCATATCCC,  downstream forward: _UP4_TAAAACTTGGACATTCAAAG

References

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